Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells
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چکیده
منابع مشابه
Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells
AIM To construct a recombinant eukaryotic expression plasmid containing human calcitonin (hCT) gene and express the gene in murine fibroblast NIH3T3 cells. MATERIALS AND METHODS A murine Igkappa-chain leader sequence and hCT gene were synthesized and cloned into pCDNA3.0 to form the pCDNA3.0-Igkappa-hCT eukaryotic expression vector, which was transfected into NIH3T3 cells. The mRNA and protei...
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Prolyl hydroxylase domain 3 (PHD3) is a hypoxia inducible factor-α (HIFα) regulator; it degrades HIFα in the presence of oxygen. Recently, there have been an increasing number of studies about the role of PHD3 in proliferation and apoptosis of cancer cells. However, most of the evidence for the role of PHD3 is observational, and little is known of the molecular mechanism. In our current study, ...
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Background: Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging. Methods: In this study, PCR-based site-directed mutagen...
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ژورنال
عنوان ژورنال: World Journal of Gastroenterology
سال: 2005
ISSN: 1007-9327
DOI: 10.3748/wjg.v11.i2.182